EENdb - TALEN/ZFN  >  ZFN detail
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ZN0087
IDZN0087
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitecGCGATCACCaagtacGCAGATCTAa
gCGCTAGTGGttcatgCGTCTAGATt
LengthLeft+[Spacer]+Right: 3F+[6]+3F
Speicessilk worm (Bombyx mori)
List EENs targeting genome of silk worm
Gene / LocusBmBLOS2 G
Location to geneexon
ZFNs Left ZFP(s)ZP10547 (F1->Fn): HTGHLLE TSGNLVR TSGHLVR
Target (5'->3'): GGTGATCGC
Right ZFP(s)ZP10546 (F1->Fn): QNSTLTE TSGNLVR QSGDLTR
Target (5'->3'): GCAGATCTA
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: TGQK

More about ZFN frameworks & linker peptides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction strategyMA
List EENs constructed by MA   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, reporter gene disruption assay & direct sequencing.
More about efficiency detection methods
Heritability
Specificity
CommentsFull sequence provided.
Reference (first reported)Takasu et al., Insect Biochem Mol Biol., 2010 (20692340 R)




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