EENdb - TALEN/ZFN  >  ZFN detail
Summary: ZFNs related to ZN0081.
IDWhole SiteSpecies & LocusZFP-L.ZFP-R.Ref.Effic.
ZN0081 gGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
mouse, Abcb1a (Mdr1a) G, exon G ZP10553 ZP10554 20628038 R
ZN0081B gGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
rat, Abcb1a (Mdr1a) G, exon G ZP10553 ZP10554 20628038 R


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ZN0081   ^ Top
IDZN0081
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitegGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
LengthLeft+[Spacer]+Right: 4F+[6]+5F
Speicesmouse (Mus musculus)
List EENs targeting genome of mouse
Gene / LocusAbcb1a (Mdr1a) G
Location to geneexon
ZFNs Left ZFP(s)ZP10553 (F1->Fn): DRSHLSR TSGNLTR QSSDLSR RSDHLTQ
Target (5'->3'): AGGGCTGATGGC
Right ZFP(s)ZP10554 (F1->Fn): TSGHLSR QSSDLSR QSADRTK RSDVLSE QSGHLSR
Target (5'->3'): GGACTGTCAGCTGGT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay. Large deletion (248-731 bp), direct-PCR.
HR addition by 21151125.
More about efficiency detection methods
Heritability
SpecificityAbcb1b and other 19 portential off-targets tested and none showed cleavage.
Comments7-aa sequences are provided by 21151125.
Reference (first reported)Carbery et al., Genetics., 2010 (20628038 R)


ZN0081B   ^ Top
IDZN0081B
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitegGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
LengthLeft+[Spacer]+Right: 4F+[6]+5F
Speicesrat (Rattus norvegicus)
List EENs targeting genome of rat
Gene / LocusAbcb1a (Mdr1a) G
Location to geneexon
ZFNs Left ZFP(s)ZP10553 (F1->Fn): DRSHLSR TSGNLTR QSSDLSR RSDHLTQ
Target (5'->3'): AGGGCTGATGGC
Right ZFP(s)ZP10554 (F1->Fn): TSGHLSR QSSDLSR QSADRTK RSDVLSE QSGHLSR
Target (5'->3'): GGACTGTCAGCTGGT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay. Large deletion (248-731 bp), direct-PCR.
HR addition by 21151125.
More about efficiency detection methods
Heritability
SpecificityAbcb1b and other 19 portential off-targets tested and none showed cleavage.
Comments7-aa sequences are provided by 21151125.
Reference (first reported)Carbery et al., Genetics., 2010 (20628038 R)




User comments   ^ Top

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