EENdb - TALEN/ZFN  >  ZFN detail
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ZN0075
IDZN0075
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full siteaCCAACCTCACTATGCactataGGTATGAGAAGGGGGa
tGGTTGGAGTGATACGtgatatCCATACTCTTCCCCCt
LengthLeft+[Spacer]+Right: 5F+[6]+5F
Speicesrat (Rattus norvegicus)
List EENs targeting genome of rat
Gene / LocusIl2rg G
Location to geneexon-intron
ZFNs Left ZFP(s)ZPU0005 (F1->Fn): (N/A) (N/A) (N/A) (N/A) (N/A)
Target (5'->3'): GCAATAGTGAGGTTGG
Right ZFP(s)ZPU0006 (F1->Fn): (N/A) (N/A) (N/A) (N/A) (N/A)
Target (5'->3'): GGTATGAGAAGGGGG
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+ (see ref.), direct sequencing.
More about efficiency detection methods
Heritability
Specificity16 portential off-targets tested and none showed cleavage.
Comments
Reference (first reported)Mashimo et al., PLoS One., 2010 (20111598 R)




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