EENdb - TALEN/ZFN  >  ZFN detail
Summary: ZFNs related to ZN0054.
IDWhole SiteSpecies & LocusZFP-L.ZFP-R.Ref.Effic.
ZN0054 cGTGCACCAAAACTTCTCCtcccacTACCGGGCCACCATTGGTg
gCACGTGGTTTTGAAGAGGagggtgATGGCCCGGTGGTAACCAc
rat, Rab38 G, exon G ZP10462 ZP10463 19628861 R
ZN0054-T002 gCACCAAAACTTCTCCtcgcacTACCGGGCCACCATTGTg
cGTGGTTTTGAAGAGGagcgtgATGGCCCGGTGGTAACAc
mouse, Rab38 G, exon G ZP10462 ZP10463 22660928 R
ZN0054B gCACCAAAACTTCTCCtcgcacTACCGGGCCACCATTGTg
cGTGGTTTTGAAGAGGagcgtgATGGCCCGGTGGTAACAc
mouse, Rab38 G, exon G ZPU0057 ZPU0058 22660928 R


User comments


ZN0054   ^ Top
IDZN0054
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitecGTGCACCAAAACTTCTCCtcccacTACCGGGCCACCATTGGTg
gCACGTGGTTTTGAAGAGGagggtgATGGCCCGGTGGTAACCAc
LengthLeft+[Spacer]+Right: 6F+[6]+6F
Speicesrat (Rattus norvegicus)
List EENs targeting genome of rat
Gene / LocusRab38 G
Location to geneexon
ZFNs Left ZFP(s)ZP10462 (F1->Fn): DRSNLSS RSHSLLR RSDSLSA TSGSLTR QSGNLAR QSGHLSR
Target (5'->3'): GGAGAAGTTTTGGTGCAC
Right ZFP(s)ZP10463 (F1->Fn): TSGHLSR HKWQRNK DRSVLRR DSSTRKK RSDHLSE DKSNRKK
Target (5'->3'): TACCGGGCCACCATTGGT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Geurts et al., Science., 2009 (19628861 R)


ZN0054-T002   ^ Top
IDZN0054-T002
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitegCACCAAAACTTCTCCtcgcacTACCGGGCCACCATTGTg
cGTGGTTTTGAAGAGGagcgtgATGGCCCGGTGGTAACAc
LengthLeft+[Spacer]+Right: 6F+[6]+6F
Speicesmouse (Mus musculus)
List EENs targeting genome of mouse
Gene / LocusRab38 G
Location to geneexon
ZFNs Left ZFP(s)ZP10462 (F1->Fn): DRSNLSS RSHSLLR RSDSLSA TSGSLTR QSGNLAR QSGHLSR
Target (5'->3'): GGAGAAGTTTTGGTGCAC
Right ZFP(s)ZP10463 (F1->Fn): TSGHLSR HKWQRNK DRSVLRR DSSTRKK RSDHLSE DKSNRKK
Target (5'->3'): TACCGGGCCACCATTGGT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
EfficiencyNHEJ: +, restriction-enzyme-resistance assay (after PCR) (BsaJI). HR: 3.5%, Southern blotting.
More about efficiency detection methods
Heritability
Specificity
CommentsUse ssODN as HR donor.
Reference (first reported)Meyer et al., Proc Natl Acad Sci U S A., 2012 (22660928 R)


ZN0054B   ^ Top
IDZN0054B
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitegCACCAAAACTTCTCCtcgcacTACCGGGCCACCATTGTg
cGTGGTTTTGAAGAGGagcgtgATGGCCCGGTGGTAACAc
LengthLeft+[Spacer]+Right: 5F+[6]+(2+5F)
Speicesmouse (Mus musculus)
List EENs targeting genome of mouse
Gene / LocusRab38 G
Location to geneexon
ZFNs Left ZFP(s)ZPU0057 (F1->Fn): (N/A) (N/A) (N/A) (N/A) (N/A)
Target (5'->3'): GGAGAAGTTTTGGTG
Right ZFP(s)ZPU0058 (F1->Fn): (N/A) (N/A) (N/A) (N/A) (N/A)
Target (5'->3'): TACCGGGCCACCATTGT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: (N/A)

More about ZFN frameworks & linker peptides
FokI variant(N/A)
More about FokI variants
Construction strategy(N/A)
More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
EfficiencyNHEJ: +, restriction-enzyme-resistance assay (after PCR) (BsaJI). HR: 3.5%, Southern blotting.
More about efficiency detection methods
Heritability
Specificity
CommentsUse ssODN as HR donor.
Reference (first reported)Meyer et al., Proc Natl Acad Sci U S A., 2012 (22660928 R)




User comments   ^ Top

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