EENdb - TALEN/ZFN  >  ZFN detail
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ZN0053
IDZN0053
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitecGAGAGCCCCCTGTCTgatgagAATTTGGTGGCCATGGGCt
gCTCTCGGGGGACAGActactcTTAAACCACCGGTACCCGa
LengthLeft+[Spacer]+Right: 5F+[6]+6F
Speicesrat (Rattus norvegicus)
List EENs targeting genome of rat
Gene / LocusIGG2B (IgM) G
Location to geneexon
ZFNs Left ZFP(s)ZP10460 (F1->Fn): NKVGLIE TSSDLSR RSDHLSR RSDNLSE QNAHRKT
Target (5'->3'): AGACAGGGGGCTCTC
Right ZFP(s)ZP10461 (F1->Fn): DRSHLTR RSDALTQ DRSDLSR RSDALAR RSDSLSA TSSNRKT
Target (5'->3'): AATTTGGTGGCCATGGGC
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity8 portential off-targets tested and none showed cleavage.
Comments
Reference (first reported)Geurts et al., Science., 2009 (19628861 R)




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