EENdb - TALEN/ZFN  >  TALEN detail
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TNT10
IDTNT10
Type / formZFN, . ()
More about ZFN forms
Target Full sitetGCACTTGTCCCGGCCTgtaaaggattgtggagATTGTACCCCACAAATa
aCGTGAACAGGGCCGGAcatttcctaacacctcTAACATGGGGTGTTTAt
LengthLeft+[Spacer]+Right: 16+[16]+16
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusdre-mir-92a-1 G
Location to gene3'-downstream
TALENs Left site & RVDs
NN HD NI HD NG NG NN NG HD HD HD NN NN HD HD NG
  G  C  A  C  T  T  G  T  C  C  C  G  G  C  C  T  
Right site & RVDs
NI NG NG NG NN NG NN NN NN NN NG NI HD NI NI NG
  A  T  T  T  G  T  G  G  G  G  T  A  C  A  A  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantSharkey-AS+R
List EENs with FokI variants Sharkey   More about FokI variants
Construction methodUA
List EENs constructed by UA   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, NHEJ-DisDel
More about modification methods
Efficiency75%, restriction-enzyme-resistance assay (after PCR).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)T1
External database
& resource




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