EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA048.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA048 tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
(artificial) 22+[x]+28 (std.) 23028976 R
TNA048B tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
(artificial) 22+[x]+28 NK 23028976 R

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TNA048   ^ Top
IDTNA048
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
LengthLeft+[Spacer]+Right: 22+[x]+28
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NN NN HD HD NG NN HD HD NN NN HD HD HD NG NN NN HD HD NN NN NG
  C  G  G  C  C  T  G  C  C  G  G  C  C  C  T  G  G  C  C  G  G  T  
Right site & RVDs
HD HD HD NN HD NN HD HD NG HD NI NN NN NN HD HD HD NN NN HD HD NN HD HD NI HD HD NG
  C  C  C  G  C  G  C  C  T  C  A  G  G  G  C  C  C  G  G  C  C  G  C  C  A  C  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments351+352
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA048B   ^ Top
IDTNA048B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
LengthLeft+[Spacer]+Right: 22+[x]+28
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NK NK HD HD NG NK HD HD NK NK HD HD HD NG NK NK HD HD NK NK NG
  C  G  G  C  C  T  G  C  C  G  G  C  C  C  T  G  G  C  C  G  G  T  
Right site & RVDs
HD HD HD NK HD NK HD HD NG HD NI NK NK NK HD HD HD NK NK HD HD NK HD HD NI HD HD NG
  C  C  C  G  C  G  C  C  T  C  A  G  G  G  C  C  C  G  G  C  C  G  C  C  A  C  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments351+352 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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