EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA047.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA047 tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
(artificial) 19+[x]+18 (std.) 23028976 R
TNA047B tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
(artificial) 19+[x]+18 NK 23028976 R

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TNA047   ^ Top
IDTNA047
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
LengthLeft+[Spacer]+Right: 19+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN HD NG NG NN HD NG NG NI HD HD NG NN NI HD HD NG NI NG
  G  C  T  T  G  C  T  T  A  C  C  T  G  A  C  C  T  A  T  
Right site & RVDs
HD NG NN NI NI NI NI HD NI NI NI NI NI NG NI NG NN NG
  C  T  G  A  A  A  A  C  A  A  A  A  A  T  A  T  G  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments349+350
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA047B   ^ Top
IDTNA047B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
LengthLeft+[Spacer]+Right: 19+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK HD NG NG NK HD NG NG NI HD HD NG NK NI HD HD NG NI NG
  G  C  T  T  G  C  T  T  A  C  C  T  G  A  C  C  T  A  T  
Right site & RVDs
HD NG NK NI NI NI NI HD NI NI NI NI NI NG NI NG NK NG
  C  T  G  A  A  A  A  C  A  A  A  A  A  T  A  T  G  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments349+350 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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