EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA046.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA046 tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
(artificial) 21+[x]+18 (std.) 23028976 R
TNA046B tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
(artificial) 21+[x]+18 NK 23028976 R No

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TNA046   ^ Top
IDTNA046
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
LengthLeft+[Spacer]+Right: 21+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN HD NN NI NI NI NN NI NN NG HD HD NI HD HD NI HD HD NI NG
  G  G  C  G  A  A  A  G  A  G  T  C  C  A  C  C  A  C  C  A  T  
Right site & RVDs
NI HD NN NI HD NI NG NN NN HD NG NG NN NI NI HD NG NG
  A  C  G  A  C  A  T  G  G  C  T  T  G  A  A  C  T  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments312+313
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA046B   ^ Top
IDTNA046B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
LengthLeft+[Spacer]+Right: 21+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NK HD NK NI NI NI NK NI NK NG HD HD NI HD HD NI HD HD NI NG
  G  G  C  G  A  A  A  G  A  G  T  C  C  A  C  C  A  C  C  A  T  
Right site & RVDs
NI HD NK NI HD NI NG NK NK HD NG NG NK NI NI HD NG NG
  A  C  G  A  C  A  T  G  G  C  T  T  G  A  A  C  T  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments312+313 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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