EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA045.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA045 tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
(artificial) 20+[x]+17 (std.) 23028976 R
TNA045B tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
(artificial) 20+[x]+17 NK 23028976 R Low

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TNA045   ^ Top
IDTNA045
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
LengthLeft+[Spacer]+Right: 20+[x]+17
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NG HD HD NI NI NI NI NN NI NN HD NN NN NG HD NG NN NI NG
  A  T  C  C  A  A  A  A  G  A  G  C  G  G  T  C  T  G  A  T  
Right site & RVDs
NN HD NI NI HD NI NI NG HD HD NG NN NI NN NN NI NG
  G  C  A  A  C  A  A  T  C  C  T  G  A  G  G  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments308+309
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA045B   ^ Top
IDTNA045B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
LengthLeft+[Spacer]+Right: 20+[x]+17
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NG HD HD NI NI NI NI NK NI NK HD NK NK NG HD NG NK NI NG
  A  T  C  C  A  A  A  A  G  A  G  C  G  G  T  C  T  G  A  T  
Right site & RVDs
NK HD NI NI HD NI NI NG HD HD NG NK NI NK NK NI NG
  G  C  A  A  C  A  A  T  C  C  T  G  A  G  G  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyLow (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments308+309 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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