EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA044.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA044 tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
(artificial) 24+[x]+18 (std.) 23028976 R
TNA044B tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
(artificial) 24+[x]+18 NK 23028976 R No

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TNA044   ^ Top
IDTNA044
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
LengthLeft+[Spacer]+Right: 24+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NN HD NG HD NI NN NG HD NI NG HD NI NI NI NN NN HD NI NI NI NN HD NG
  A  G  C  T  C  A  G  T  C  A  T  C  A  A  A  G  G  C  A  A  A  G  C  T  
Right site & RVDs
NN NG NI NI HD HD NG HD HD NI NN NI NN HD HD NI NN NG
  G  T  A  A  C  C  T  C  C  A  G  A  G  C  C  A  G  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments304+305
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA044B   ^ Top
IDTNA044B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
LengthLeft+[Spacer]+Right: 24+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NK HD NG HD NI NK NG HD NI NG HD NI NI NI NK NK HD NI NI NI NK HD NG
  A  G  C  T  C  A  G  T  C  A  T  C  A  A  A  G  G  C  A  A  A  G  C  T  
Right site & RVDs
NK NG NI NI HD HD NG HD HD NI NK NI NK HD HD NI NK NG
  G  T  A  A  C  C  T  C  C  A  G  A  G  C  C  A  G  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments304+305 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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