EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA043.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA043 tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
(artificial) 20+[x]+20 (std.) 23028976 R
TNA043B tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
(artificial) 20+[x]+20 NK 23028976 R No

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TNA043   ^ Top
IDTNA043
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
LengthLeft+[Spacer]+Right: 20+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NG NI HD HD NG NG HD NI NG NG NN NI NG NN NG HD HD HD NG
  C  T  A  C  C  T  T  C  A  T  T  G  A  T  G  T  C  C  C  T  
Right site & RVDs
NI NN NI NN NN NI NN HD NG NG NG NN HD HD NG NG NG HD NI NG
  A  G  A  G  G  A  G  C  T  T  T  G  C  C  T  T  T  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments300+301
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA043B   ^ Top
IDTNA043B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
LengthLeft+[Spacer]+Right: 20+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NG NI HD HD NG NG HD NI NG NG NK NI NG NK NG HD HD HD NG
  C  T  A  C  C  T  T  C  A  T  T  G  A  T  G  T  C  C  C  T  
Right site & RVDs
NI NK NI NK NK NI NK HD NG NG NG NK HD HD NG NG NG HD NI NG
  A  G  A  G  G  A  G  C  T  T  T  G  C  C  T  T  T  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments300+301 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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