EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA042.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA042 tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
(artificial) 17+[x]+21 (std.) 23028976 R Low
TNA042B tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
(artificial) 17+[x]+21 NK 23028976 R No

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TNA042   ^ Top
IDTNA042
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
LengthLeft+[Spacer]+Right: 17+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NG NG NG NI HD NI NI NN NN NI NN HD NI HD NN NG
  C  T  T  T  A  C  A  A  G  G  A  G  C  A  C  G  T  
Right site & RVDs
NN NG HD NI HD NN HD NG NI NI NI HD NG HD HD NG NG NG NI NI NG
  G  T  C  A  C  G  C  T  A  A  A  C  T  C  C  T  T  T  A  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyLow (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments282+283
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA042B   ^ Top
IDTNA042B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
LengthLeft+[Spacer]+Right: 17+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD NG NG NG NI HD NI NI NK NK NI NK HD NI HD NK NG
  C  T  T  T  A  C  A  A  G  G  A  G  C  A  C  G  T  
Right site & RVDs
NK NG HD NI HD NK HD NG NI NI NI HD NG HD HD NG NG NG NI NI NG
  G  T  C  A  C  G  C  T  A  A  A  C  T  C  C  T  T  T  A  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments282+283 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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