EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA040.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA040 tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
(artificial) 16+[x]+21 (std.) 23028976 R
TNA040-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
(artificial) 19+[x]+26 others 23028976 R No
TNA040B tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
(artificial) 16+[x]+21 NK 23028976 R No
TNA040B-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
(artificial) 19+[x]+26 others 23028976 R No
TNA040C tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
(artificial) 16+[x]+21 NK 23028976 R
TNA040C-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
(artificial) 19+[x]+26 others 23028976 R
TNA040D tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
(artificial) 16+[x]+21 NK 23028976 R
TNA040D-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
(artificial) 19+[x]+26 others 23028976 R No
TNA040E tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
(artificial) 16+[x]+21 NK 23028976 R No
TNA040E-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
(artificial) 19+[x]+26 others 23028976 R No

User comments


TNA040   ^ Top
IDTNA040
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
LengthLeft+[Spacer]+Right: 16+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  G  G  A  G  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  G  T  T  C  A  T  A  A  A  A  A  C  T  G  G  G  C  G  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272+273
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040-T002   ^ Top
IDTNA040-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
LengthLeft+[Spacer]+Right: 19+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  A  A  A  A  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  A  T  T  C  A  T  A  A  A  A  A  C  T  A  A  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272 + 273C-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040B   ^ Top
IDTNA040B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
LengthLeft+[Spacer]+Right: 16+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NK NI NK NI NI NG NG HD HD HD NI HD NG NG NG
  G  G  A  G  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NK NG NG HD NI NG NI NI NI NI NI HD NG NK NK NK HD NK NI HD NG
  G  T  T  C  A  T  A  A  A  A  A  C  T  G  G  G  C  G  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272+273 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040B-T002   ^ Top
IDTNA040B-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
LengthLeft+[Spacer]+Right: 19+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NK NI NK NI NI NG NG HD HD HD NI HD NG NG NG
  A  A  A  A  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NK NG NG HD NI NG NI NI NI NI NI HD NG NK NK NK HD NK NI HD NG
  A  T  T  C  A  T  A  A  A  A  A  C  T  A  A  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272N-NK + 273
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040C   ^ Top
IDTNA040C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
LengthLeft+[Spacer]+Right: 16+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NK NI NK NI NI NG NG HD HD HD NI HD NG NG NG
  G  G  A  G  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  G  T  T  C  A  T  A  A  A  A  A  C  T  G  G  G  C  G  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272 + 273N-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040C-T002   ^ Top
IDTNA040C-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
LengthLeft+[Spacer]+Right: 19+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NK NI NK NI NI NG NG HD HD HD NI HD NG NG NG
  A  A  A  A  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  A  T  T  C  A  T  A  A  A  A  A  C  T  A  A  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272 + 273C-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040D   ^ Top
IDTNA040D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
LengthLeft+[Spacer]+Right: 16+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  G  G  A  G  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NK NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  G  T  T  C  A  T  A  A  A  A  A  C  T  G  G  G  C  G  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272+273
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040D-T002   ^ Top
IDTNA040D-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
LengthLeft+[Spacer]+Right: 19+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  A  A  A  A  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NK NG NG HD NI NG NI NI NI NI NI HD NG NN NN NN HD NN NI HD NG
  A  T  T  C  A  T  A  A  A  A  A  C  T  A  A  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272+273 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040E   ^ Top
IDTNA040E
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
LengthLeft+[Spacer]+Right: 16+[x]+21
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  G  G  A  G  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NK NK NK HD NK NI HD NG
  G  T  T  C  A  T  A  A  A  A  A  C  T  G  G  G  C  G  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272N-NK + 273
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA040E-T002   ^ Top
IDTNA040E-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
LengthLeft+[Spacer]+Right: 19+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NN NI NN NI NI NG NG HD HD HD NI HD NG NG NG
  A  A  A  A  A  A  T  T  C  C  C  A  C  T  T  T  
Right site & RVDs
NN NG NG HD NI NG NI NI NI NI NI HD NG NK NK NK HD NK NI HD NG
  A  T  T  C  A  T  A  A  A  A  A  C  T  A  A  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments272 + 273N-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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