EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA039.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA039 tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
(artificial) 19+[x]+27 (std.) 23028976 R Low
TNA039B tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
(artificial) 19+[x]+27 NK 23028976 R No

User comments


TNA039   ^ Top
IDTNA039
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
LengthLeft+[Spacer]+Right: 19+[x]+27
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD HD NG NG NG NG NI NI NN NI NI NN NG NG NN NN HD NI NG
  C  C  T  T  T  T  A  A  G  A  A  G  T  T  G  G  C  A  T  
Right site & RVDs
NI NN NI NN NI HD HD HD NI NN NN NG NG NG NG NI NI NI NG NG NN NG NI NG NG NI NG
  A  G  A  G  A  C  C  C  A  G  G  T  T  T  T  A  A  A  T  T  G  T  A  T  T  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyLow (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments270+271
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA039B   ^ Top
IDTNA039B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
LengthLeft+[Spacer]+Right: 19+[x]+27
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD HD NG NG NG NG NI NI NK NI NI NK NG NG NK NK HD NI NG
  C  C  T  T  T  T  A  A  G  A  A  G  T  T  G  G  C  A  T  
Right site & RVDs
NI NK NI NK NI HD HD HD NI NK NK NG NG NG NG NI NI NI NG NG NK NG NI NG NG NI NG
  A  G  A  G  A  C  C  C  A  G  G  T  T  T  T  A  A  A  T  T  G  T  A  T  T  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments270+271 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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