EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA037.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA037 tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
(artificial) 16+[x]+17 (std.) 23028976 R
TNA037B tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
(artificial) 16+[x]+17 NK 23028976 R

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TNA037   ^ Top
IDTNA037
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
LengthLeft+[Spacer]+Right: 16+[x]+17
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD HD NI NG NG HD HD NG NI NG NN NI HD NG NN NG
  C  C  A  T  T  C  C  T  A  T  G  A  C  T  G  T  
Right site & RVDs
NI NG NI HD NG HD NI HD NI HD NI NI NG NI NN HD NG
  A  T  A  C  T  C  A  C  A  C  A  A  T  A  G  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments143+144
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA037B   ^ Top
IDTNA037B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
LengthLeft+[Spacer]+Right: 16+[x]+17
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD HD NI NG NG HD HD NG NI NG NK NI HD NG NK NG
  C  C  A  T  T  C  C  T  A  T  G  A  C  T  G  T  
Right site & RVDs
NI NG NI HD NG HD NI HD NI HD NI NI NG NI NK HD NG
  A  T  A  C  T  C  A  C  A  C  A  A  T  A  G  C  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast & HEK293 cells.
More about efficiency detection methods
Heritability
Specificity
Comments143+144 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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