EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA036.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA036 tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 (std.) 23028976 R
TNA036-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R No
TNA036B tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 NK 23028976 R Low
TNA036B-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R
TNA036C tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 NK 23028976 R
TNA036C-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R
TNA036D tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 NK 23028976 R
TNA036D-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R
TNA036E tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 NK 23028976 R No
TNA036E-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R
TNA036F tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
(artificial) 15+[x]+16 NK 23028976 R
TNA036F-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
(artificial) 18+[x]+20 others 23028976 R Low

User comments


TNA036   ^ Top
IDTNA036
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166+167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036-T002   ^ Top
IDTNA036-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166 + 167C-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036B   ^ Top
IDTNA036B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NG HD NK NG HD NG NG HD NG NK HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NK NG HD NI NK HD NI HD HD NI NK NK HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyLow (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166+167 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036B-T002   ^ Top
IDTNA036B-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NG HD NK NG HD NG NG HD NG NK HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NK NG HD NI NK HD NI HD HD NI NK NK HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166N-NK + 167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036C   ^ Top
IDTNA036C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NG HD NK NG HD NG NG HD NG NN HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166C-NK + 167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036C-T002   ^ Top
IDTNA036C-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NK NG HD NK NG HD NG NG HD NG NN HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166 + 167N-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036D   ^ Top
IDTNA036D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NK HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166 + 167C-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036D-T002   ^ Top
IDTNA036D-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NK HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NN NN HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166+167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036E   ^ Top
IDTNA036E
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NK NG HD NI NK HD NI HD HD NI NN NN HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency- (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166+167 NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036E-T002   ^ Top
IDTNA036E-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NK NG HD NI NK HD NI HD HD NI NN NN HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166N-NK + 167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036F   ^ Top
IDTNA036F
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
LengthLeft+[Spacer]+Right: 15+[x]+16
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  G  T  C  G  T  C  T  T  C  T  G  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NK NK HD NI NG
  A  G  T  C  A  G  C  A  C  C  A  G  G  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166C-NK + 167
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource


TNA036F-T002   ^ Top
IDTNA036F-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
LengthLeft+[Spacer]+Right: 18+[x]+20
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN NG HD NN NG HD NG NG HD NG NN HD NI HD NG
  A  T  C  A  T  C  T  T  C  T  A  C  A  C  T  
Right site & RVDs
NI NN NG HD NI NN HD NI HD HD NI NK NK HD NI NG
  A  A  T  C  A  A  C  A  C  C  A  A  A  C  A  T  
TALEN framework
& linker peptide
+231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyLow (see ref.), SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments166 + 167N-NK
Reference (first reported)Christian et al., PLoS One., 2012 (23028976 R)
External database
& resource




User comments   ^ Top

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