EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA031.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA031 tATAATTAATAATCCACTTn(x)AAGTGGATTATTAATTATa
aTATTAATTATTAGGTGAAn(x)TTCACCTAATAATTAATAt
(artificial) 18+[x]+18 NS-N 21813459 R
TNA031B tATAATTAATAATCCACTTaggctcgagttacAGAGGGTTAGGTTTATAGa
aTATTAATTATTAGGTGAAtccgagctcaatgTCTCCCAATCCAAATATCt
(artificial) 18+[13]+18 NS-N 21813459 R

User comments


TNA031   ^ Top
IDTNA031
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATAATTAATAATCCACTTn(x)AAGTGGATTATTAATTATa
aTATTAATTATTAGGTGAAn(x)TTCACCTAATAATTAATAt
LengthLeft+[Spacer]+Right: 18+[x]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NG NI NI NG NG NI NS NG NI NS NG HD HD NS HD NG NG
  A  T  A  A  T  T  A  A  T  A  A  T  C  C  A  C  T  T  
Right site & RVDs
NI NG NI NI NG NG NI NS NG NI NS NG HD HD NS HD NG NG
  A  T  A  A  T  T  A  A  T  A  A  T  C  C  A  C  T  T  
TALEN framework
& linker peptide
AvrBs4-BB, AvrBs4-NH, AvrBs4-EH, AvrBs4-EC, AvrBs4-NC
More about TALEN frameworks & linker pepetides
FokI variantKV+EA
List EENs with FokI variants KV+EA   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+, in vitro cleavage testing & reporter gene correction assay, in HEK293T cells
More about efficiency detection methods
Heritability
Specificity
Comments"AvrBs4-x-AvrBs4".
x=6,8,9,10,12,15,16,18,21,27, see Table S1.
Different spacer length showed different activity with special linker (C-terminus of framework).
Reference (first reported)Mussolino et al., Nucleic Acids Res., 2011 (21813459 R)
External database
& resource


TNA031B   ^ Top
IDTNA031B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATAATTAATAATCCACTTaggctcgagttacAGAGGGTTAGGTTTATAGa
aTATTAATTATTAGGTGAAtccgagctcaatgTCTCCCAATCCAAATATCt
LengthLeft+[Spacer]+Right: 18+[13]+18
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI NG NI NI NG NG NI NS NG NI NS NG HD HD NS HD NG NG
  A  T  A  A  T  T  A  A  T  A  A  T  C  C  A  C  T  T  
Right site & RVDs
HD NG NS NG NI NI NI HD HD NG NS NS HD HD HD NG HD NG
  C  T  A  T  A  A  A  C  C  T  A  A  C  C  C  T  C  T  
TALEN framework
& linker peptide
+17 AvrBs4-NH
More about TALEN frameworks & linker pepetides
FokI variantKV+EA
List EENs with FokI variants KV+EA   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+, in vitro cleavage testing & reporter gene correction assay, in HEK293T cells.
More about efficiency detection methods
Heritability
Specificity
Comments"AvrBs4-13-AvrBs3".
Reference (first reported)Mussolino et al., Nucleic Acids Res., 2011 (21813459 R)
External database
& resource




User comments   ^ Top

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