EENdb - TALEN/ZFN  >  TALEN detail
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TNA010
IDTNA010
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCCTTTATCTCTcgaatcccgggtacgaAGAGATAAAGGGa
aGGGAAATAGAGAgcttagggcccatgctTCTCTATTTCCCt
LengthLeft+[Spacer]+Right: 12+[16]+12
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
HD HD HD NG NG NG NS NG HD NG HD NG
  C  C  C  T  T  T  A  T  C  T  C  T  
Right site & RVDs
HD HD HD NG NG NG NS NG HD NG HD NG
  C  C  C  T  T  T  A  T  C  T  C  T  
TALEN framework
& linker peptide
+286 Hax3
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(unnamed)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+ (see ref.), in vitro cleavage testing & SSA assay in tobacco.
More about efficiency detection methods
Heritability
Specificity
Comments"dHax3.N".
Spacer = 10 bp showed low activity and spacer = 6 bp showed no activity.
Reference (first reported)Mahfouz et al., Proc Natl Acad Sci U S A., 2011 (21262818 R)
External database
& resource




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