EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA008.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA008
(T-M)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
(artificial) 26 N*; NS-N; others 20699274 R
TNA008B
(T-M-N)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
(artificial) 26 N*; NS-N; others 20699274 R
TNA008C
(T-D-N)
TTAGCACCTGGTTGGAGGGGGTTTATan(x)tATAAACCCCCTCCAACCAGGTGCTAA
AATCGTGGACCAACCTCCCCCAAATAtn(x)aTATTTGGGGGAGGTTGGTCCACGATT
(artificial) 26+[x+2]+26 N*; NS-N; others 20699274 R
TNA008D tATAAACCCCCTCCAACCAGGTGCTAAn(x)TTAGCACCTGGTTGGAGGGGGTTTATa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)AATCGTGGACCAACCTCCCCCAAATAt
(artificial) 26+[x]+26 N*; NS-N; others 20699274 R
TNA008E/TNA007C tATAAACCCCCTCCAACCAGGTGCTAAn(x)GTGGTGTACAGTAGGGGGAGATGCa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)CACCACATGTCATCCCCCTCTACGt
(artificial) 26+[x]+24 N*; NS-N; others 20699274 R
TNA008F tGCATCTCCCCCTACTGTACACCACcaaaagtgaattcatgagcTTAGCACCTGGTTGGAGGGGGTTTATa
aCGTAGAGGGGGATGACATGTGGTGgttttcacttaagtactcgAATCGTGGACCAACCTCCCCCAAATAt
(artificial) 24+[19]+26 N*; NS-N; others 21459844 R

User comments


TNA008   ^ Top
IDTNA008
Type / formTALEN, monomer. (T-M)
More about TALEN forms
Target Full sitetATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
Length26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  A  T  A  A  A  C  C  C  C  C  T  C  C  A  A  C  C  A  G  G  T  G  C  T  A  A  
Right site & RVDs
TALEN framework
& linker peptide
+297 AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"AvrXa7-FN".
TALEN monomer, from AvrXa7 (295aa+2aa).
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource


TNA008B   ^ Top
IDTNA008B
Type / formTALEN, monomer, with FokI domain in N-terminus. (T-M-N)
More about TALEN forms
Target Full sitetATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
Length26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  A  T  A  A  A  C  C  C  C  C  T  C  C  A  A  C  C  A  G  G  T  G  C  T  A  A  
Right site & RVDs
TALEN framework
& linker peptide
4+288(N) AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"FN-AvrXa7".
TALEN monomer, from AvrXa7; FokI in N-terminus
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource


TNA008C   ^ Top
IDTNA008C
Type / formTALEN, dimer, with FokI domain in N-terminus. (T-D-N)
More about TALEN forms
Target Full siteTTAGCACCTGGTTGGAGGGGGTTTATan(x)tATAAACCCCCTCCAACCAGGTGCTAA
AATCGTGGACCAACCTCCCCCAAATAtn(x)aTATTTGGGGGAGGTTGGTCCACGATT
LengthLeft+[Spacer]+Right: 26+[x+2]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  T  T  A  G  C  A  C  C  T  G  G  T  T  G  G  A  G  G  G  G  G  T  T  T  A  T  
Right site & RVDs(Right half-target & TALE RVDs: same as Left)
TALEN framework
& linker peptide
4+288(N) AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"FN-AvrXa7 + FN-AvrXa7".
x=6,14,19,24,30,35,40; all showed weak cleavage.
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource


TNA008D   ^ Top
IDTNA008D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATAAACCCCCTCCAACCAGGTGCTAAn(x)TTAGCACCTGGTTGGAGGGGGTTTATa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)AATCGTGGACCAACCTCCCCCAAATAt
LengthLeft+[Spacer]+Right: 26+[x]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  A  T  A  A  A  C  C  C  C  C  T  C  C  A  A  C  C  A  G  G  T  G  C  T  A  A  
Right site & RVDs(Right half-target & TALE RVDs: same as Left)
TALEN framework
& linker peptide
+297 AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"AvrXa7-FN + AvrXa7-FN".
x=2,5,8,19; x=19 showed efficient cleavage.
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource


TNA008E/TNA007C   ^ Top
IDTNA008E/TNA007C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATAAACCCCCTCCAACCAGGTGCTAAn(x)GTGGTGTACAGTAGGGGGAGATGCa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)CACCACATGTCATCCCCCTCTACGt
LengthLeft+[Spacer]+Right: 26+[x]+24
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  A  T  A  A  A  C  C  C  C  C  T  C  C  A  A  C  C  A  G  G  T  G  C  T  A  A  
Right site & RVDs
NN HD NI HG HD NG N* HD HD NI NG NG NI HD NG NN NG NI NI NI NI N* NS N*
  G  C  A  T  C  T  C  C  C  C  C  T  A  C  T  G  T  A  C  A  C  C  A  C  
TALEN framework
& linker peptide
+297 AvrXa7, +297 PthXo1
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"AvrXa7-FN + PthXo1-FN".
x=6,11,16,21,26,31,36; x=16-31 showed efficient cleavage.
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource


TNA008F   ^ Top
IDTNA008F
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGCATCTCCCCCTACTGTACACCACcaaaagtgaattcatgagcTTAGCACCTGGTTGGAGGGGGTTTATa
aCGTAGAGGGGGATGACATGTGGTGgttttcacttaagtactcgAATCGTGGACCAACCTCCCCCAAATAt
LengthLeft+[Spacer]+Right: 24+[19]+26
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN HD NI HG HD NG N* HD HD NI NG NG NI HD NG NN NG NI NI NI NI N* NS N*
  G  C  A  T  C  T  C  C  C  C  C  T  A  C  T  G  T  A  C  A  C  C  A  C  
Right site & RVDs
NI HG NI NI NS HD NN HD HD HD NS N* N* HD HD NS NS NN NN NI NG NN NI N* NS N*
  A  T  A  A  A  C  C  C  C  C  T  C  C  A  A  C  C  A  G  G  T  G  C  T  A  A  
TALEN framework
& linker peptide
+297 AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(Y)
List EENs constructed by GG / Y /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
EfficiencyHR 8.5-16%, SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments"PthXo1-FN + AvrXa7-FN"; with a in-frame length including ATG.
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (21459844 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TNA008 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us