EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TNA007.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TNA007 (N/A) (artificial) 24+[13-30]+24 20660643 R
TNA007B
(T-M)
tGCATCTCCCCCTACTGTACACCAC
aCGTAGAGGGGGATGACATGTGGTG
(artificial) 24 NS-N; others 20699274 R

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TNA007   ^ Top
IDTNA007
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full site(N/A)
LengthLeft+[Spacer]+Right: 24+[13-30]+24
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN HD NI HG HD NG N* HD HD NI NG NG NI HD NG NN NG NI NI NI NI N* NS N*
(target unknown)
Right site & RVDs(Right half-target & TALE RVDs: same as Left)
TALEN framework
& linker peptide
+235 PthXo1
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+, SSA assay, in yeast.
More about efficiency detection methods
Heritability
Specificity
Comments"PthXo1", target site consist of two of the natural targets (unspecified in this article) of PthXo1 and spacer n(x).
x=12-30; x=15 showed highest efficiency.
Reference (first reported)Christian et al., Genetics., 2010 (20660643 R)
External database
& resource


TNA007B   ^ Top
IDTNA007B
Type / formTALEN, monomer. (T-M)
More about TALEN forms
Target Full sitetGCATCTCCCCCTACTGTACACCAC
aCGTAGAGGGGGATGACATGTGGTG
Length24
Speices(artificial)
List EENs targeting genome of (artificial)
Gene / Locus
Location to gene
TALENs Left site & RVDs
NN HD NI HG HD NG N* HD HD NI NG NG NI HD NG NN NG NI NI NI NI N* NS N*
  G  C  A  T  C  T  C  C  C  C  C  T  A  C  T  G  T  A  C  A  C  C  A  C  
Right site & RVDs
TALEN framework
& linker peptide
+297 PthXo1
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency+; in vitro cleavage testing
More about efficiency detection methods
Heritability
Specificity
Comments"PthXo1-FN".
TALEN monomer, from PthXo1 (295aa+2aa).
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (20699274 R)
External database
& resource




User comments   ^ Top

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