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TN0207
IDTN0207
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCACCAAGTACGCAGATCTaaggagcctagccgcgAACCTGAACAAGACCCTTa
aGTGGTTCATGCGTCTAGAttcctcggatcggcgcTTGGACTTGTTCTGGGAAt
LengthLeft+[Spacer]+Right: 18+[16]+18
Speicessilk worm (Bombyx mori)
List EENs targeting genome of silk worm
Gene / LocusBmBLOS2 G
Location to geneexon
TALENs Left site & RVDs
HD NI HD HD NI NI NN NG NI HD NN HD NI NN NI NG HD NG
  C  A  C  C  A  A  G  T  A  C  G  C  A  G  A  T  C  T  
Right site & RVDs
NI NI NN NN NN NG HD NG NG NN NG NG HD NI NN NN NG NG
  A  A  G  G  G  T  C  T  T  G  T  T  C  A  G  G  T  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction methodGG-PCR
List EENs constructed by GG / PCR   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, NHEJ-DisDel
More about modification methods
EfficiencyNHEJ: +, reporter gene disruption assay. NHEJ-DisDel with TN0206, direct PCR.
More about efficiency detection methods
Heritability
Specificity
CommentsThe referece named these FokI variants as "NEL+CKK".
Reference (first reported)Ma et al., PLoS One., 2012 (23028749 R)
External database
& resource




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