EENdb - TALEN/ZFN  >  TALEN detail
User comments


TN0206
IDTN0206
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGATCCACATGATCCTGTcatcagtcggttagccACACAGCTCTTTAAAAAAa
aCCTAGGTGTACTAGGACAgtagtcagccaatcggTGTGTCGAGAAATTTTTTt
LengthLeft+[Spacer]+Right: 18+[16]+18
Speicessilk worm (Bombyx mori)
List EENs targeting genome of silk worm
Gene / LocusBmBLOS2 G
Location to geneexon
TALENs Left site & RVDs
NN NN NI NG HD HD NI HD NI NG NN NI NG HD HD NG NN NG
  G  G  A  T  C  C  A  C  A  T  G  A  T  C  C  T  G  T  
Right site & RVDs
NG NG NG NG NG NG NI NI NI NN NI NN HD NG NN NG NN NG
  T  T  T  T  T  T  A  A  A  G  A  G  C  T  G  T  G  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction methodGG-PCR
List EENs constructed by GG / PCR   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, NHEJ-DisDel
More about modification methods
EfficiencyNHEJ: +, reporter gene disruption assay. NHEJ-DisDel with TN0207, direct PCR.
More about efficiency detection methods
Heritability
Specificity
CommentsThe referece named these FokI variants as "NEL+CKK".
Reference (first reported)Ma et al., PLoS One., 2012 (23028749 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0206 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us