EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0199.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0199A tGTGATGAACACTCCACAGAATCTcgatgctgtcgttaccCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAgctacgacagcaatggGAGATTGACACCTAAAACTt
cow, MSTN (GDF8) G, exon G 23+[16]+19 (std.) 23027955 R
TN0199B tGTGATGAACACTCCACAGAATCTcgatgctgtcgttaccctctaACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAgctacgacagcaatgggagatTGACACCTAAAACTTCGAAAACCt
cow, MSTN (GDF8) G, exon G 23+[21]+23 (std.) 23027955 R No

User comments


TN0199A   ^ Top
IDTN0199A
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTGATGAACACTCCACAGAATCTcgatgctgtcgttaccCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAgctacgacagcaatggGAGATTGACACCTAAAACTt
LengthLeft+[Spacer]+Right: 23+[16]+19
Speicescow (Bos taurus)
List EENs targeting genome of cow
Gene / LocusMSTN (GDF8) G
Location to geneexon
TALENs Left site & RVDs
NN NG NN NI NG NN NI NI HD NI HD NG HD HD NI HD NI NN NI NI NG HD NG
  G  T  G  A  T  G  A  A  C  A  C  T  C  C  A  C  A  G  A  A  T  C  T  
Right site & RVDs
NG HD NI NI NI NI NG HD HD NI HD NI NN NG NG NI NN NI NN
  T  C  A  A  A  A  T  C  C  A  C  A  G  T  T  A  G  A  G  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency50%, direct sequencing, in embryos. 19%, mismatch-detection nuclease assay, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"btGDF83.1L+83.1NR"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource


TN0199B   ^ Top
IDTN0199B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTGATGAACACTCCACAGAATCTcgatgctgtcgttaccctctaACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAgctacgacagcaatgggagatTGACACCTAAAACTTCGAAAACCt
LengthLeft+[Spacer]+Right: 23+[21]+23
Speicescow (Bos taurus)
List EENs targeting genome of cow
Gene / LocusMSTN (GDF8) G
Location to geneexon
TALENs Left site & RVDs
NN NG NN NI NG NN NI NI HD NI HD NG HD HD NI HD NI NN NI NI NG HD NG
  G  T  G  A  T  G  A  A  C  A  C  T  C  C  A  C  A  G  A  A  T  C  T  
Right site & RVDs
HD HD NI NI NI NI NN HD NG NG HD NI NI NI NI NG HD HD NI HD NI NN NG
  C  C  A  A  A  A  G  C  T  T  C  A  A  A  A  T  C  C  A  C  A  G  T  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency-, mismatch-detection nuclease assay, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"btGDF83.1L+83.1R"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0199 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us