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TN0196
IDTN0196
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCCTTTTTAGAAGTCAAGGTaacagacacaccaaaAAGATCCAGGAGAGATTTTGGa
aGGGAAAAATCTTCAGTTCCAttgtctgtgtggtttTTCTAGGTCCTCTCTAAAACCt
LengthLeft+[Spacer]+Right: 20+[15]+21
Speicespig (Sus scrofa)
List EENs targeting genome of pig
Gene / LocusMSTN (GDF8) G
Location to geneexon
TALENs Left site & RVDs
HD HD HD NG NG NG NG NG NI NN NI NI NN NG HD NI NI NN NN NG
  C  C  C  T  T  T  T  T  A  G  A  A  G  T  C  A  A  G  G  T  
Right site & RVDs
HD HD NI NI NI NI NG HD NG HD NG HD HD NG NN NN NI NG HD NG NG
  C  C  A  A  A  A  T  C  T  C  T  C  C  T  G  G  A  T  C  T  T  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency19.2%, mismatch-detection nuclease assay, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"ssGDF8E3.1+83.1NR"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource




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