EENdb - TALEN/ZFN  >  TALEN detail
User comments


TN0185
IDTN0185
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGCAATCCCAGGCTTCACCGTTgagccagaaaacaagACGGAATGGGAACTTGCCTACa
aCGTTAGGGTCCGAAGTGGCAActcggtcttttgttcTGCCTTACCCTTGAACGGATGt
LengthLeft+[Spacer]+Right: 21+[15]+21
Speicescow (Bos taurus)
List EENs targeting genome of cow
Gene / LocusACAN G
Location to geneintron-exon
TALENs Left site & RVDs
NN HD NI NI NG HD HD HD NI NN NN HD NG NG HD NI HD HD NN NG NG
  G  C  A  A  T  C  C  C  A  G  G  C  T  T  C  A  C  C  G  T  T  
Right site & RVDs
NN NG NI NN NN HD NI NI NN NG NG HD HD HD NI NG NG HD HD NN NG
  G  T  A  G  G  C  A  A  G  T  T  C  C  C  A  T  T  C  C  G  T  
TALEN framework
& linker peptide
+63-Goldy, +231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method

More about modification methods
Efficiency<1%, direct sequencing, in embryos. -, mismatch-detection nuclease assay, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"btACAN11L+11R"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0185 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us