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TN0177
IDTN0177
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTATACCTAGGTCAAAAATgtaatgaagaatatcATGGCTGGATTGCAACAAACTa
aGATATGGATCCAGTTTTTAcattacttcttatagTACCGACCTAACGTTGTTTGAt
LengthLeft+[Spacer]+Right: 19+[15]+21
Speicespig (Sus scrofa)
List EENs targeting genome of pig
Gene / LocusDMD G
Location to geneintron-exon
TALENs Left site & RVDs
HD NG NI NG NI HD HD NG NI NN NN NG HD NI NI NI NI NI NG
  C  T  A  T  A  C  C  T  A  G  G  T  C  A  A  A  A  A  T  
Right site & RVDs
NI NN NG NG NG NN NG NG NN HD NI NI NG HD HD NI NN HD HD NI NG
  A  G  T  T  T  G  T  T  G  C  A  A  T  C  C  A  G  C  C  A  T  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, NHEJ-DisDel, NHEJ-Inv
More about modification methods
EfficiencyNHEJ: 24-25%, mismatch-detection nuclease assay, in fibroblasts. NHEJ-DisDel/NHEJ-Inv with TN0176, direct PCR, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"DMD6L+6R"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource




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