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TN0172
IDTN0172
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATATGAAACTGACAGTATtaatggcctgaacctAGCCAGAACTCAGATTGGGa
aTATACTTTGACTGTCATAattaccggacttggaTCGGTCTTGAGTCTAACCCt
LengthLeft+[Spacer]+Right: 18+[15]+19
Speicespig (Sus scrofa)
List EENs targeting genome of pig
Gene / LocusSRY G
Location to gene3'-downstream 1.5kb
TALENs Left site & RVDs
NI NG NI NG NN NI NI NI HD NG NN NI HD NI NN NG NI NG
  A  T  A  T  G  A  A  A  C  T  G  A  C  A  G  T  A  T  
Right site & RVDs
HD HD HD NI NI NG HD NG NN NI NN NG NG HD NG NN NN HD NG
  C  C  C  A  A  T  C  T  G  A  G  T  T  C  T  G  G  C  T  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency20%, mismatch-detection nuclease assay, in fibroblasts.
More about efficiency detection methods
Heritability
Specificity
Comments"ssSRY ds3.3"
Reference (first reported)Carlson et al., Proc Natl Acad Sci U S A., 2012 (23027955 R)
External database
& resource




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