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TN0169
IDTN0169
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTCCTCAACATACATACTggagagttagttgacaAAGATGAATCATTTGTa
aGAGGAGTTGTATGTATGAcctctcaatcaactgtTTCTACTTAGTAAACAt
LengthLeft+[Spacer]+Right: 18+[16]+16
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locuscdh5 G
Location to geneexon
TALENs Left site & RVDs
HD NG HD HD NG HD NI NI HD NI NG NI HD NI NG NI HD NG
  C  T  C  C  T  C  A  A  C  A  T  A  C  A  T  A  C  T  
Right site & RVDs
NI HD NI NI NI NG NN NI NG NG HD NI NG HD NG NG
  A  C  A  A  A  T  G  A  T  T  C  A  T  C  T  T  
TALEN framework
& linker peptide
+63-Goldy
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency100%, restriction-enzyme-resistance assay (after PCR) (HincII).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Bedell et al., Nature., 2012 (23000899 R)
External database
& resource




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