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TN0166
IDTN0166
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetTGCAACACTGAGCTCTGTAAACCTgtccgcgtgtggcgaACCTCAGGGTCCAGTCGGCAGCAGa
aACGTTGTGACTCGAGACATTTGGAcaggcgcacaccgctTGGAGTCCCAGGTCAGCCGTCGTCt
LengthLeft+[Spacer]+Right: 24+[15]+24
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locuscrhr1 G
Location to geneupstream
TALENs Left site & RVDs
NG NN HD NI NI HD NI HD NG NN NI NN HD NG HD NG NN NG NI NI NI HD HD NG
  T  G  C  A  A  C  A  C  T  G  A  G  C  T  C  T  G  T  A  A  A  C  C  T  
Right site & RVDs
HD NG NN HD NG NN HD HD NN NI HD NG NN NN NI HD HD HD NG NN NI NN NN NG
  C  T  G  C  T  G  C  C  G  A  C  T  G  G  A  C  C  C  T  G  A  G  G  T  
TALEN framework
& linker peptide
+63-Goldy, +231
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency6-35%/0-3% (+63-Goldy/+231), restriction-enzyme-resistance assay (after PCR) (BstUI).
More about efficiency detection methods
Heritability
Specificity
CommentsSlight different to Ensembl.
Reference (first reported)Bedell et al., Nature., 2012 (23000899 R)
External database
& resource




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