EENdb - TALEN/ZFN  >  TALEN detail
User comments


TN0162
IDTN0162
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
LengthLeft+[Spacer]+Right: 18+[15]+17
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusryr3 G
Location to geneexon
TALENs Left site & RVDs
NN NN NI HD NI NI NG NG HD NI NG HD HD NN NN HD NI NG
  G  G  A  C  A  A  T  T  C  A  T  C  C  G  G  C  A  T  
Right site & RVDs
NN HD NN HD NI HD HD NG NG HD NG HD NI HD HD HD NG
  G  C  G  C  A  C  C  T  T  C  T  C  A  C  C  C  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantD+R
List EENs with FokI variants D+R   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, HRMA.
More about efficiency detection methods
Heritability
SpecificityTarget sites of ryr1a, ryr1b tested and none showed activity.
Comments
Reference (first reported)Dahlem et al., PLoS Genet., 2012 (22916025 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0162 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us