EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0150.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
zebrafish, epas1b G, exon G 17+[17]+17 (std.) 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
zebrafish, epas1b [mutated] G, exon G 17+[17]+17 (std.) 22684503 R

User comments


TN0150   ^ Top
IDTN0150
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
LengthLeft+[Spacer]+Right: 17+[17]+17
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusepas1b G
Location to geneexon
TALENs Left site & RVDs
NI HD NI NI NG NI HD NG HD HD HD NI HD NG NN NI NI
  A  C  A  A  T  A  C  T  C  C  C  A  C  T  G  A  A  
Right site & RVDs
NI HD HD NI NI HD NG NN NG HD HD NI NG NN NI NN NG
  A  C  C  A  A  C  T  G  T  C  C  A  T  G  A  G  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT; ELD+KKR
List EENs with FokI variants WT; ELD+KKR   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency76% (WT FokI) or 58% (ELD+KKR FokI), direct sequencing.
More about efficiency detection methods
Heritability
SpecificityA variant target locus with a 2 base pair mismatch showed activity.
Commentsepas1b.2
Reference (first reported)Cade et al., Nucleic Acids Res., 2012 (22684503 R)
External database
& resource


TN0150-T002   ^ Top
IDTN0150-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
LengthLeft+[Spacer]+Right: 17+[17]+17
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusepas1b [mutated] G
Location to geneexon
TALENs Left site & RVDs
NI HD NI NI NG NI HD NG HD HD HD NI HD NG NN NI NI
  A  C  A  A  T  A  C  T  C  C  C  A  C  T  G  A  A  
Right site & RVDs
NI HD HD NI NI HD NG NN NG HD HD NI NG NN NI NN NG
  A  C  C  A  A  C  T  G  T  C  C  A  T  G  A  G  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT; ELD+KKR
List EENs with FokI variants WT; ELD+KKR   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency2.2% (WT FokI) or 2.6% (ELD+KKR FokI), direct sequencing.
More about efficiency detection methods
Heritability
SpecificityOff-target
Comments
Reference (first reported)Cade et al., Nucleic Acids Res., 2012 (22684503 R)
External database
& resource




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0150 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us