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TN0135
IDTN0135
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTGGATCGCGGAGGGAAtgccccggagggcggaGAACTGGGACGAGGCCGa
aGACCTAGCGCCTCCCTTacggggcctcccgcctCTTGACCCTGCTCCGGCt
LengthLeft+[Spacer]+Right: 17+[16]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusVHL G
Location to genearound ATG
TALENs Left site & RVDs
HD NG NN NN NI NG HD NN HD NN NN NI NN NN NN NI NI
  C  T  G  G  A  T  C  G  C  G  G  A  G  G  G  A  A  
Right site & RVDs
HD NN NN HD HD NG HD NN NG HD HD HD NI NN NG NG HD
  C  G  G  C  C  T  C  G  T  C  C  C  A  G  T  T  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency(see ref.), mismatch-detection nuclease assay (T7E1).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Reyon et al., Nat Biotechnol., 2012 (22484455 R)
External database
& resource
Addgene 36845+36846




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