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TN0133
IDTN0133
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACa
aACGGCAGGGTTCGTTACctactaaactacgacagGGGCCTGCTATAACTTGt
LengthLeft+[Spacer]+Right: 17+[17]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusTP53 G
Location to geneexon
TALENs Left site & RVDs
NG NN HD HD NN NG HD HD HD NI NI NN HD NI NI NG NN
  T  G  C  C  G  T  C  C  C  A  A  G  C  A  A  T  G  
Right site & RVDs
NN NG NG HD NI NI NG NI NG HD NN NG HD HD NN NN NN
  G  T  T  C  A  A  T  A  T  C  G  T  C  C  G  G  G  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency(see ref.), mismatch-detection nuclease assay (T7E1).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Reyon et al., Nat Biotechnol., 2012 (22484455 R)
External database
& resource
Addgene 36768+36853




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