EENdb - TALEN/ZFN  >  TALEN detail
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TN0101
IDTN0101
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTa
aGTGACAGCCGCCGGTACtgtggcgagcagagggcgGACTCAGCAGACACCCAt
LengthLeft+[Spacer]+Right: 17+[18]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusMUTYH G
Location to genearound ATG
TALENs Left site & RVDs
HD NI HD NG NN NG HD NN NN HD NN NN HD HD NI NG NN
  C  A  C  T  G  T  C  G  G  C  G  G  C  C  A  T  G  
Right site & RVDs
NI HD HD HD NI HD NI NN NI HD NN NI HD NG HD NI NN
  A  C  C  C  A  C  A  G  A  C  G  A  C  T  C  A  G  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency(see ref.), mismatch-detection nuclease assay (T7E1).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Reyon et al., Nat Biotechnol., 2012 (22484455 R)
External database
& resource
Addgene 36677+36678




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