EENdb - TALEN/ZFN  >  TALEN detail
User comments


TN0083
IDTN0083
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCTCTGAGACGCCATGTtcaactcgatgaccccACCACCAATCAGTAGCTa
aGGAGACTCTGCGGTACAagttgagctactggggTGGTGGTTAGTCATCGAt
LengthLeft+[Spacer]+Right: 17+[16]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusGLI1 G
Location to genearound ATG
TALENs Left site & RVDs
HD HD NG HD NG NN NI NN NI HD NN HD HD NI NG NN NG
  C  C  T  C  T  G  A  G  A  C  G  C  C  A  T  G  T  
Right site & RVDs
NI NN HD NG NI HD NG NN NI NG NG NN NN NG NN NN NG
  A  G  C  T  A  C  T  G  A  T  T  G  G  T  G  G  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodFLASH
List EENs constructed by FLASH   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency(see ref.), mismatch-detection nuclease assay (T7E1).
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Reyon et al., Nat Biotechnol., 2012 (22484455 R)
External database
& resource
Addgene 36707+36708




User comments   ^ Top

Add a new comment
NOTE:
According to legal, governmental and/or server supplier's requirements, and in order to prevent spams, your comment might need to be reviewed by the administrator before public. Please be patient and do not submit the same content again, thank you very much. Old and archived comments might be deleted.
You can also send your comments of TN0083 to the administrator of EENdb
by e-mail.
For suggestion or comments to the whole database, please also contact us by e-mail.
Your nickname (1-16 characters, required):
Your email (1-50 characters, required): Hide email to other users
The e-mail will be shown with modifies to avoid spams for you.
Your comments (1-1500 characters, plain text only):


©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us