EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0034.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0034 tCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacaCTCAAAGACGAGAAAt
zebrafish, gria3a G, exon G 15+[17]+15 (std.) 21822241 R
TN0034B tCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacACTCAAAGACGAGAAAt
zebrafish, gria3a G, exon G 15+[16]+16 (std.) 21822241 R
TN0034C tCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAGtcagtgcgtgcggacaCTCAAAGACGAGAAAt
zebrafish, gria3a G, exon G 16+[16]+15 (std.) 21822241 R

User comments


TN0034   ^ Top
IDTN0034
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacaCTCAAAGACGAGAAAt
LengthLeft+[Spacer]+Right: 15+[17]+15
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusgria3a G
Location to geneexon
TALENs Left site & RVDs
HD NN NG HD HD NI NI NG NI NN HD NG NG HD NG
  C  G  T  C  C  A  A  T  A  G  C  T  T  C  T  
Right site & RVDs
NI NI NI NN NI NN HD NI NN NI NI NI HD NG HD
  A  A  A  G  A  G  C  A  G  A  A  A  C  T  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodREAL
List EENs constructed by REAL   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency15%, direct sequencing.
42% with EL+KK FokI, 61% with ELD+KKR FokI in 22684503.
More about efficiency detection methods
Heritability
Specificity
Comments"#1258/#1260".
Germ-line transmission by 22684503.
Reference (first reported)Sander et al., Nat Biotechnol., 2011 (21822241 R)
External database
& resource
Addgene 32281+32282


TN0034B   ^ Top
IDTN0034B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacACTCAAAGACGAGAAAt
LengthLeft+[Spacer]+Right: 15+[16]+16
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusgria3a G
Location to geneexon
TALENs Left site & RVDs
HD NN NG HD HD NI NI NG NI NN HD NG NG HD NG
  C  G  T  C  C  A  A  T  A  G  C  T  T  C  T  
Right site & RVDs
NI NI NI NN NI NN HD NI NN NI NI NI HD NG HD NI
  A  A  A  G  A  G  C  A  G  A  A  A  C  T  C  A  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodREAL
List EENs constructed by REAL   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency31%, direct sequencing.
More about efficiency detection methods
Heritability
Specificity
Comments"#1258/#1259"
Reference (first reported)Sander et al., Nat Biotechnol., 2011 (21822241 R)
External database
& resource
Addgene 32281+32284


TN0034C   ^ Top
IDTN0034C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAGtcagtgcgtgcggacaCTCAAAGACGAGAAAt
LengthLeft+[Spacer]+Right: 16+[16]+15
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locusgria3a G
Location to geneexon
TALENs Left site & RVDs
HD NN NG HD HD NI NI NG NI NN HD NG NG HD NG HD
  C  G  T  C  C  A  A  T  A  G  C  T  T  C  T  C  
Right site & RVDs
NI NI NI NN NI NN HD NI NN NI NI NI HD NG HD
  A  A  A  G  A  G  C  A  G  A  A  A  C  T  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodREAL
List EENs constructed by REAL   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency33%, direct sequencing.
More about efficiency detection methods
Heritability
Specificity
Comments"#1295/#1260"
Reference (first reported)Sander et al., Nat Biotechnol., 2011 (21822241 R)
External database
& resource
Addgene 32283+32282




User comments   ^ Top

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