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TN0033
IDTN0033
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAa
aGAAGGCAAAGGTGTAGGtggtgtagggttgtctcgTCGCCCTCGTCGTCATTt
LengthLeft+[Spacer]+Right: 17+[18]+17
Speiceszebrafish (Danio rerio)
List EENs targeting genome of zebrafish
Gene / Locushey2 G
Location to geneexon
TALENs Left site & RVDs
HD NG NG HD HD NN NG NG NG HD HD NI HD NI NG HD NG
  C  T  T  C  C  G  T  T  T  C  C  A  C  A  T  C  C  
Right site & RVDs
NG NG NI HD NG NN HD NG NN HD NG HD HD HD NN HD NG
  T  T  A  C  T  G  C  T  G  C  T  C  C  C  G  C  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodREAL
List EENs constructed by REAL   More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency11%, direct sequencing.
More about efficiency detection methods
Heritability
Specificity
Comments"#1297/#1257".
Germ-line transmission by 22684503.
Reference (first reported)Sander et al., Nat Biotechnol., 2011 (21822241 R)
External database
& resource
Addgene 32279+32280




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