EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0031.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0031 tGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
human, CCR5 G, exon G 18+[15]+18 (std.) 21813459 R
TN0031-T002 tGTGGGCAACATGCTGGTCgtcctcatcttaataAACTGCAAAAAGCTGAAGt
aCACCCGTTGTACGACCAGcaggagtagaattatTTGACGTTTTTCGACTTCa
human, CCR2 G, exon G 18+[15]+18 others 21813459 R Low

User comments


TN0031   ^ Top
IDTN0031
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
LengthLeft+[Spacer]+Right: 18+[15]+18
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NN NG NN NN NN HD NI NI HD NI NG NN HD NG NN NN NG HD
  G  T  G  G  G  C  A  A  C  A  T  G  C  T  G  G  T  C  
Right site & RVDs
HD NG NG HD NI NN HD HD NG NG NG NG NN HD NI NN NG NG
  C  T  T  C  A  G  C  C  T  T  T  T  G  C  A  G  T  T  
TALEN framework
& linker peptide
+47 AvrBs4-NC, +17 AvrBs4-NH
More about TALEN frameworks & linker pepetides
FokI variantKV+EA
List EENs with FokI variants KV+EA   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency7-17%, mismatch-detection nuclease assay (T7E1), in HEK293T cells.
More about efficiency detection methods
Heritability
SpecificityCCR2 is the off-target
Comments
Reference (first reported)Mussolino et al., Nucleic Acids Res., 2011 (21813459 R)
External database
& resource


TN0031-T002   ^ Top
IDTN0031-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGTGGGCAACATGCTGGTCgtcctcatcttaataAACTGCAAAAAGCTGAAGt
aCACCCGTTGTACGACCAGcaggagtagaattatTTGACGTTTTTCGACTTCa
LengthLeft+[Spacer]+Right: 18+[15]+18
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR2 G
Location to geneexon
TALENs Left site & RVDs
NN NG NN NN NN HD NI NI HD NI NG NN HD NG NN NN NG HD
  G  T  G  G  G  C  A  A  C  A  T  G  C  T  G  G  T  C  
Right site & RVDs
HD NG NG HD NI NN HD HD NG NG NG NG NN HD NI NN NG NG
  C  T  T  C  A  G  C  T  T  T  T  T  G  C  A  G  T  T  
TALEN framework
& linker peptide
+47 AvrBs4-NC, +17 AvrBs4-NH
More about TALEN frameworks & linker pepetides
FokI variantKV+EA
List EENs with FokI variants KV+EA   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency0-1%, mismatch-detection nuclease assay (T7E1), in HEK293T cells,
More about efficiency detection methods
Heritability
SpecificityOff-target
Comments
Reference (first reported)Mussolino et al., Nucleic Acids Res., 2011 (21813459 R)
External database
& resource




User comments   ^ Top

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