EENdb - TALEN/ZFN  >  TALEN detail
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TN0030
IDTN0030
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetACACGTTTCGTGTTCGGAgccgctttaacccacTCTGTGGAAGTGCTCAGCa
aTGTGCAAAGCACAAGCCTcggcgaaattgggtgAGACACCTTCACGAGTCGt
LengthLeft+[Spacer]+Right: 18+[15]+18
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusIL2RG G
Location to gene
TALENs Left site & RVDs
NI HD NI HD NN NG NG NG HD NN NG NN NG NG HD NN NN NI
  A  C  A  C  G  T  T  T  C  G  T  G  T  T  C  G  G  A  
Right site & RVDs
NN HD NG NN NI NN HD NI HD NG NG HD HD NI HD NI NN NI
  G  C  T  G  A  G  C  A  C  T  T  C  C  A  C  A  G  A  
TALEN framework
& linker peptide
+47 AvrBs4-NC, +17 AvrBs4-NH
More about TALEN frameworks & linker pepetides
FokI variantKV+EA
List EENs with FokI variants KV+EA   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, in vitro cleavage testing & reporter gene correction assay, in HEK293T cells
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Mussolino et al., Nucleic Acids Res., 2011 (21813459 R)
External database
& resource




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