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TN0029
IDTN0029
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTGGGCTCTCCCATgcattcaaactgaggtgcctgcCCTTCTAGGAATGGGGGa
aGACCCGAGAGGGTAcgtaagtttgactccacggacgGGAAGATCCTTACCCCCt
LengthLeft+[Spacer]+Right: 14+[22]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusPOU5F1 (OCT4) G
Location to gene3'-UTR
TALENs Left site & RVDs
HD NG NN NN NN HD NG HD NG HD HD HD NI NG
  C  T  G  G  G  C  T  C  T  C  C  C  A  T  
Right site & RVDs
HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
  C  C  C  C  C  A  T  T  C  C  T  A  G  A  A  G  G  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay & direct PCR (HR), in ES & iPS cells.
More about efficiency detection methods
Heritability
SpecificityOff-target
Comments"101138/101142".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource




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