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TN0028
IDTN0028
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCACCTGCAGCTGCCCAGacctggcacccaggagAGGAGCAGGCAGGGTCa
aGTGGACGTCGACGGGTCtggaccgtgggtcctcTCCTCGTCCGTCCCAGt
LengthLeft+[Spacer]+Right: 17+[16]+16
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusPOU5F1 (OCT4) G
Location to geneintron
TALENs Left site & RVDs
HD NI HD HD NG NN HD NI NN HD NG NN HD HD HD NI NN
  C  A  C  C  T  G  C  A  G  C  T  G  C  C  C  A  G  
Right site & RVDs
NN NI HD HD HD NG NN HD HD NG NN HD NG HD HD NG
  G  A  C  C  C  T  G  C  C  T  G  C  T  C  C  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), eGFP reporter gene & mismatch-detection nuclease assay & direct PCR (HR), in ES & iPS cells
More about efficiency detection methods
Heritability
Specificity
Comments"101123/101125".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource




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