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TN0027
IDTN0027
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetACGCCGTGGAAAGGCCcgtatgagcggccccgcccGTAGATCATCCCCGa
aTGCGGCACCTTTCCGGgcatactcgccggggcgggCATCTAGTAGGGGCt
LengthLeft+[Spacer]+Right: 16+[19]+14
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusPITX3 G
Location to gene3'-UTR
TALENs Left site & RVDs
NI HD NN HD HD NN NG NN NN NI NI NI NN NN HD HD
  A  C  G  C  C  G  T  G  G  A  A  A  G  G  C  C  
Right site & RVDs
HD NN NN NN NN NI NG NN NI NG HD NG NI HD
  C  G  G  G  G  A  T  G  A  T  C  T  A  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay & direct PCR (HR), in ES & iPS cells.
More about efficiency detection methods
Heritability
Specificity
Comments"101236/101238".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource




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