EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0025.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0025 tCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
human, PPP1R12C (AAVS1) G, intron G 17+[15]+17 (std.) 21738127 R
TN0025-T002 tTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
human, [intergenic] 17+[13]+17 others 21738127 R
TN0025-T003 cCCCCTCCACCCCAAAAAtttctaaaaacattgttttgtgaaAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTaaagatttttgtaacaaaacacttTTATACTCAATATCTTTt
human, [intergenic] 17+[24]+17 others 21738127 R

User comments


TN0025   ^ Top
IDTN0025
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
LengthLeft+[Spacer]+Right: 17+[15]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusPPP1R12C (AAVS1) G
Location to geneintron
TALENs Left site & RVDs
HD HD HD HD NG HD HD NI HD HD HD HD NI HD NI NN NG
  C  C  C  C  T  C  C  A  C  C  C  C  A  C  A  G  T  
Right site & RVDs
NG NG NG HD NG NN NG HD NI HD HD NI NI NG HD HD NG
  T  T  T  C  T  G  T  C  A  C  C  A  A  T  C  C  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT; ELD+KKR
List EENs with FokI variants WT; ELD+KKR   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay & direct PCR (HR), in ES & iPS cells.
More about efficiency detection methods
Heritability
Specificity19 candidate off-target sites tested and 2 showed activity.
Comments"101077/101079".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource


TN0025-T002   ^ Top
IDTN0025-T002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
LengthLeft+[Spacer]+Right: 17+[13]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / Locus[intergenic]
Location to gene
TALENs Left site & RVDs
HD HD HD HD NG HD HD NI HD HD HD HD NI HD NI NN NG
  T  C  C  A  C  C  C  A  G  C  C  C  T  C  A  G  T  
Right site & RVDs
NG NG NG HD NG NN NG HD NI HD HD NI NI NG HD HD NG
  T  T  C  C  A  G  C  C  A  C  C  C  A  T  C  C  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantELD+KKR
List EENs with FokI variants ELD+KKR   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay, in ES & iPS cells.
More about efficiency detection methods
Heritability
SpecificityOff-target
Comments"OT10".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource


TN0025-T003   ^ Top
IDTN0025-T003
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitecCCCCTCCACCCCAAAAAtttctaaaaacattgttttgtgaaAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTaaagatttttgtaacaaaacacttTTATACTCAATATCTTTt
LengthLeft+[Spacer]+Right: 17+[24]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / Locus[intergenic]
Location to gene
TALENs Left site & RVDs
HD HD HD HD NG HD HD NI HD HD HD HD NI HD NI NN NG
  C  C  C  C  T  C  C  A  C  C  C  C  A  A  A  A  A  
Right site & RVDs
NG NG NG HD NG NN NG HD NI HD HD NI NI NG HD HD NG
  T  T  T  C  T  A  T  A  A  C  T  C  A  T  A  T  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantELD+KKR
List EENs with FokI variants ELD+KKR   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, mismatch-detection nuclease assay, in ES & iPS cells.
More about efficiency detection methods
Heritability
SpecificityOff-target
Comments"OT14".
Reference (first reported)Hockemeyer et al., Nat Biotechnol., 2011 (21738127 R)
External database
& resource




User comments   ^ Top

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