EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0024.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0024 tgggatCCAGCCTGATGGAACttataagggagaaagtgATTTGCAGTTGGAAAGAatcaa
accctaGGTCGGACTACCTTGaatattccctctttcacTAAACGTCAACCTTTCTtagtt
worm, ben-1 G, exon G 15+[17]+17 (std.) 21700836 R
TN0024B tgggatCCAGCCTGATGGAACttataagggagaaagtgatttGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGaatattccctctttcactaaaCGTCAACCTTTCTTAGTt
worm, ben-1 G, exon G 15+[21]+17 (std.) 21700836 R No
TN0024C tGGGATCCAGCCTGATggaacttataagggagaaagtgATTTGCAGTTGGAAAGAatcaa
aCCCTAGGTCGGACTAccttgaatattccctctttcacTAAACGTCAACCTTTCTtagtt
worm, ben-1 G, exon G 15+[22]+17 (std.) 21700836 R No
TN0024D tgggatCCAGCCTGATGGAACTTataagggagaaagtgatttGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGAAtattccctctttcactaaaCGTCAACCTTTCTTAGTt
worm, ben-1 G, exon G 17+[19]+17 (std.) 21700836 R No

User comments


TN0024   ^ Top
IDTN0024
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgggatCCAGCCTGATGGAACttataagggagaaagtgATTTGCAGTTGGAAAGAatcaa
accctaGGTCGGACTACCTTGaatattccctctttcacTAAACGTCAACCTTTCTtagtt
LengthLeft+[Spacer]+Right: 15+[17]+17
Speicesworm (Caenorhabditis elegans)
List EENs targeting genome of worm
Gene / Locusben-1 G
Location to geneexon
TALENs Left site & RVDs
HD HD NI NN HD HD NG NN NI NG NN NN NI NI HD
  C  C  A  G  C  C  T  G  A  T  G  G  A  A  C  
Right site & RVDs
NG HD NG NG NG HD HD NI NI HD NG NN HD NI NI NI NG
  T  C  T  T  T  C  C  A  A  C  T  G  C  A  A  A  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
EfficiencyRelatively higher activity, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity
Comments"101318/101321".
Reference (first reported)Wood et al., Science., 2011 (21700836 R)
External database
& resource


TN0024B   ^ Top
IDTN0024B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgggatCCAGCCTGATGGAACttataagggagaaagtgatttGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGaatattccctctttcactaaaCGTCAACCTTTCTTAGTt
LengthLeft+[Spacer]+Right: 15+[21]+17
Speicesworm (Caenorhabditis elegans)
List EENs targeting genome of worm
Gene / Locusben-1 G
Location to geneexon
TALENs Left site & RVDs
HD HD NI NN HD HD NG NN NI NG NN NN NI NI HD
  C  C  A  G  C  C  T  G  A  T  G  G  A  A  C  
Right site & RVDs
NG NN NI NG NG HD NG NG NG HD HD NI NI HD NG NN HD
  T  G  A  T  T  C  T  T  T  C  C  A  A  C  T  G  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
EfficiencyRelatively lower activity, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity
Comments"101318/101322".
Reference (first reported)Wood et al., Science., 2011 (21700836 R)
External database
& resource


TN0024C   ^ Top
IDTN0024C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGGGATCCAGCCTGATggaacttataagggagaaagtgATTTGCAGTTGGAAAGAatcaa
aCCCTAGGTCGGACTAccttgaatattccctctttcacTAAACGTCAACCTTTCTtagtt
LengthLeft+[Spacer]+Right: 15+[22]+17
Speicesworm (Caenorhabditis elegans)
List EENs targeting genome of worm
Gene / Locusben-1 G
Location to geneexon
TALENs Left site & RVDs
NN NN NN NI NG HD HD NI NN HD HD NG NN NI NG
  G  G  G  A  T  C  C  A  G  C  C  T  G  A  T  
Right site & RVDs
NG HD NG NG NG HD HD NI NI HD NG NN HD NI NI NI NG
  T  C  T  T  T  C  C  A  A  C  T  G  C  A  A  A  T  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
EfficiencyRelatively lower activity, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity
Comments"101317/101321".
Reference (first reported)Wood et al., Science., 2011 (21700836 R)
External database
& resource


TN0024D   ^ Top
IDTN0024D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgggatCCAGCCTGATGGAACTTataagggagaaagtgatttGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGAAtattccctctttcactaaaCGTCAACCTTTCTTAGTt
LengthLeft+[Spacer]+Right: 17+[19]+17
Speicesworm (Caenorhabditis elegans)
List EENs targeting genome of worm
Gene / Locusben-1 G
Location to geneexon
TALENs Left site & RVDs
HD HD NI NN HD HD NG NN NI NG NN NN NI NI HD NG NG
  C  C  A  G  C  C  T  G  A  T  G  G  A  A  C  T  T  
Right site & RVDs
NG NN NI NG NG HD NG NG NG HD HD NI NI HD NG NN HD
  T  G  A  T  T  C  T  T  T  C  C  A  A  C  T  G  C  
TALEN framework
& linker peptide
+63
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
(Inactive)
More about modification methods
Efficiency-, mismatch-detection nuclease assay.
More about efficiency detection methods
Heritability
Specificity
Comments"101319/101322".
Reference (first reported)Wood et al., Science., 2011 (21700836 R)
External database
& resource




User comments   ^ Top

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