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TN0021
IDTN0021
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCGGATGCTCCTCTTgacaaggtctgtattgtcAGTTGTGGTTTGTCTa
aGGCCTACGAGGAGAActgttccagacataacagTCAACACCAAACAGAt
LengthLeft+[Spacer]+Right: 15+[18]+15
Speicesthale cress (Arabidopsis thaliana)
List EENs targeting genome of thale cress
Gene / LocusADH1 G
Location to geneexon
TALENs Left site & RVDs
HD HD NN NN NI NG NN HD NG HD HD NG HD NG NG
  C  C  G  G  A  T  G  C  T  C  C  T  C  T  T  
Right site & RVDs
NI NN NI HD NI NI NI HD HD NI HD NI NI HD NG
  A  G  A  C  A  A  A  C  C  A  C  A  A  C  T  
TALEN framework
& linker peptide
+231 PthXo1
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+, SSA assay, in yeast & restriction-enzyme-resistance assay (before PCR), in Arabidopsis protoplast.
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Cermak et al., Nucleic Acids Res., 2011 (21493687 R)
External database
& resource




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