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TN0010
IDTN0010
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCCATTCCTATGACTGTAGATtttatcagactgaagAGCTATTGTGTGAGTATa
aGGTAAGGATACTGACATCTAaaatagtctgacttcTCGATAACACACTCATAt
LengthLeft+[Spacer]+Right: 20+[15]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusHPRT1 G
Location to geneexon
TALENs Left site & RVDs
HD HD NI NG NG HD HD NG NI NG NN NI HD NG NN NG NI NN NI NG
  C  C  A  T  T  C  C  T  A  T  G  A  C  T  G  T  A  G  A  T  
Right site & RVDs
NI NG NI HD NG HD NI HD NI HD NI NI NG NI NN HD NG
  A  T  A  C  T  C  A  C  A  C  A  A  T  A  G  C  T  
TALEN framework
& linker peptide
+231 PthXo1
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(V&B)
List EENs constructed by GG / V / B /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+, SSA assay, in yeast & restriction-enzyme-resistance assay (before PCR), in HEK293T cells.
More about efficiency detection methods
Heritability
Specificity
Comments"HPRT1 B".
Reference (first reported)Cermak et al., Nucleic Acids Res., 2011 (21493687 R)
External database
& resource




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