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TN0004
IDTN0004
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
LengthLeft+[Spacer]+Right: 17+[17]+19
Speicesyeast (Saccharomyces cerevisiae)
List EENs targeting genome of yeast
Gene / LocusURA3 G
Location to geneexon
TALENs Left site & RVDs
NI HG NI NI NN NN NI NI HD NN HG NN HD HG NN HD N*
  A  T  A  A  G  G  A  A  C  G  T  G  C  T  G  C  T  
Right site & RVDs
NI HG HG NI NI NI HG NI NN HD HG HG NN NN HD NI NN HD N*
  A  T  T  A  A  A  T  A  G  C  T  T  G  G  C  A  G  C  A  
TALEN framework
& linker peptide
+297 AvrXa7
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction methodGG(Y)
List EENs constructed by GG / Y /    More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
EfficiencyHR 27-34%, SSA assay, in yeast & reporter gene disruption assay.
More about efficiency detection methods
Heritability
Specificity
Comments"U3a-L + U3a-R"
Reference (first reported)Li et al., Nucleic Acids Res., 2011 (21459844 R)
External database
& resource




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