EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0003.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0003 tGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
human, CCR5 G, exon G 15+[13]+15 (std.) 21179091 R
TN0003B tgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
human, CCR5 G, exon G 15+[12]+13 (std.) 21179091 R
TN0003C tgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
human, CCR5 G, exon G 15+[10]+15 (std.) 21179091 R
TN0003D tgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
human, CCR5 G, exon G 17+[12]+14 (std.) 21179091 R

User comments


TN0003   ^ Top
IDTN0003
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
LengthLeft+[Spacer]+Right: 15+[13]+15
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NN HD NG NN NN NG HD NI NG HD HD NG HD NI NG
  G  C  T  G  G  T  C  A  T  C  C  T  C  A  T  
Right site & RVDs
NN HD NG HD NG NG HD NI NN HD HD NG NG NG NG
  G  C  T  C  T  T  C  A  G  C  C  T  T  T  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 1 of 16: "L161"+"R175" (+63/+28).
See Fig.S9.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0003B   ^ Top
IDTN0003B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
LengthLeft+[Spacer]+Right: 15+[12]+13
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NN NN NG HD NI NG HD HD NG HD NI NG HD HD NG
  G  G  T  C  A  T  C  C  T  C  A  T  C  C  T  
Right site & RVDs
NN HD NG HD NG NG HD NI NN HD HD NG NG
  G  C  T  C  T  T  C  A  G  C  C  T  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 2 of 16: "L164"+"R177" (+63/+28).
See Fig.S9.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0003C   ^ Top
IDTN0003C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
LengthLeft+[Spacer]+Right: 15+[10]+15
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
HD NI NG HD HD NG HD NI NG HD HD NG NN NI NG
  C  A  T  C  C  T  C  A  T  C  C  T  G  A  T  
Right site & RVDs
HD NI NG NN HD NG HD NG NG HD NI NN HD HD NG
  C  A  T  G  C  T  C  T  T  C  A  G  C  C  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 3 of 16: "L167"+"R178" (+63/+28).
See Fig.S9.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0003D   ^ Top
IDTN0003D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
LengthLeft+[Spacer]+Right: 17+[12]+14
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
HD HD NG HD NI NG HD HD NG NN NI NG NI NI NI HD NG
  C  C  T  C  A  T  C  C  T  G  A  T  A  A  A  C  T  
Right site & RVDs
NN NG HD NI NN NG HD NI NG NN HD NG HD NG
  G  T  C  A  G  T  C  A  T  G  C  T  C  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, HR
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 4 of 16: "L172"+"R185" (+63/+28).
See Fig.S9.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource




User comments   ^ Top

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