EENdb - TALEN/ZFN  >  TALEN detail
Summary: TALENs related to TN0002.
IDWhole SiteSpecies & LocusLengthRVDsRef.Effic.
TN0002 tCTTCATTACACCTgcagctctcattttccATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAcgtcgagagtaaaaggTATGTCAGTCAtagttaagaccttctt
human, CCR5 G, exon G 13+[16]+11 (std.) 21179091 R
TN0002B tctTCATTACACCTGCAGCTctcattttccatACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAgagtaaaaggtaTGTCAGTCATAGTtaagaccttctt
human, CCR5 G, exon G 17+[12]+13 (std.) 21179091 R
TN0002C tcttcattACACCTGCAGCTCTcattttccatacagtcAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAgtaaaaggtatgtcagTCATAGTTAAGACCTTCtt
human, CCR5 G, exon G 14+[16]+17 (std.) 21179091 R
TN0002D tcttcattACACCTGCAGCTCTCATtttccatacagtcagtATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAaaaggtatgtcagtcaTAGTTAAGACCTTCTt
human, CCR5 G, exon G 17+[16]+15 (std.) 21179091 R

User comments


TN0002   ^ Top
IDTN0002
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetCTTCATTACACCTgcagctctcattttccATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAcgtcgagagtaaaaggTATGTCAGTCAtagttaagaccttctt
LengthLeft+[Spacer]+Right: 13+[16]+11
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
HD NG NG HD NI NG NG NI HD NI HD HD NG
  C  T  T  C  A  T  T  A  C  A  C  C  T  
Right site & RVDs
NI HD NG NN NI HD NG NN NG NI NG
  A  C  T  G  A  C  T  G  T  A  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 1 of 16: "L532"+"R549" (+63/+28).
See Fig.4 & S8.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0002B   ^ Top
IDTN0002B
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetctTCATTACACCTGCAGCTctcattttccatACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAgagtaaaaggtaTGTCAGTCATAGTtaagaccttctt
LengthLeft+[Spacer]+Right: 17+[12]+13
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NG HD NI NG NG NI HD NI HD HD NG NN HD NI NN HD NG
  T  C  A  T  T  A  C  A  C  C  T  G  C  A  G  C  T  
Right site & RVDs
NG NN NI NG NI HD NG NN NI HD NG NN NG
  T  G  A  T  A  C  T  G  A  C  T  G  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 2 of 16: "L538"+"R551" (+63/+28).
See Fig.4 & S8.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0002C   ^ Top
IDTN0002C
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetcttcattACACCTGCAGCTCTcattttccatacagtcAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAgtaaaaggtatgtcagTCATAGTTAAGACCTTCtt
LengthLeft+[Spacer]+Right: 14+[16]+17
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NI HD NI HD HD NG NN HD NI NN HD NG HD NG
  A  C  A  C  C  T  G  C  A  G  C  T  C  T  
Right site & RVDs
HD NG NG HD HD NI NN NI NI NG NG NN NI NG NI HD NG
  C  T  T  C  C  A  G  A  A  T  T  G  A  T  A  C  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 3 of 16: "L540"+"R557" (+63/+28).
See Fig.4 & S8.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource


TN0002D   ^ Top
IDTN0002D
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitetcttcattACACCTGCAGCTCTCATtttccatacagtcagtATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAaaaggtatgtcagtcaTAGTTAAGACCTTCTt
LengthLeft+[Spacer]+Right: 17+[16]+15
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusCCR5 G
Location to geneexon
TALENs Left site & RVDs
NI HD NI HD HD NG NN HD NI NN HD NG HD NG HD NI NG
  A  C  A  C  C  T  G  C  A  G  C  T  C  T  C  A  T  
Right site & RVDs
NG HD NG NG HD HD NI NN NI NI NG NG NN NI NG
  T  C  T  T  C  C  A  G  A  A  T  T  G  A  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+ (see ref.), mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsPair groups example 4 of 16: "L543"+"R560" (+63/+28).
See Fig.4 & S8.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource




User comments   ^ Top

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